Structural Requirements for the Action of Neurohypophyseal Hormones upon the Isolated Amphibian Urinary Bladder

Author:

Rasmussen Howard1,Schwartz Irving L.1,Young Richard1,Marc-Aurele Julien1

Affiliation:

1. From the Department of Biochemistry, University of Wisconsin, Madison, Wisconsin; the Medical Research Center, Brookhaven National Laboratory, Upton, Long Island, New York; and the Department of Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio.

Abstract

The response of the isolated amphibian urinary bladder to thirty-four structural analogs of arginine vasotocin was determined in an effort to define the physiological significance of specific structural groups on the hormone molecule. All but one of the analogs tested possessed full intrinsic activity in this system but varied greatly in their affinity for the receptor site. An analysis of the effect of changes in hydrogen ion concentration upon the response of the bladder to oxytocin was performed in order to determine the number and nature of the ionizable groups involved in hormone receptor interaction. Two ionizable groups with apparent pK's of 7.1 and 7.75 were found to be important in determining the magnitude of the hormonal response. On the basis of the results it was postulated that hormone-receptor interaction can be considered a two-step process: (a) The binding or attachment of hormone to receptor site through ionic, hydrogen, and hydrophobic bonds and (b) a disulfide interchange reaction between hormonal disulfide and receptor sulfhydryl. The latter step is considered to be the reaction which initiates the chain of events leading to the observed change in permeability.

Publisher

Rockefeller University Press

Subject

Physiology

Cited by 61 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Regulation of apical Na+ conductive transport in epithelia by pH;Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes;1995-05

2. pH dependence of the action of arginine vasopressin in renal collecting tubule;American Journal of Physiology-Renal Physiology;1992-05-01

3. Vasotocin-like activity of cerebrospinal fluid induced by habenular lesions in cats;Neuropeptides;1985-07

4. Anion transport inhibitors: effects on water and sodium transport in the toad urinary bladder;American Journal of Physiology-Renal Physiology;1985-04-01

5. Intracellular pH, transepithelial pH gradients, and ADH-induced water channels;American Journal of Physiology-Renal Physiology;1983-06-01

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