STUDIES ON CRYSTALLINE UREASE

Author:

Tauber Henry1,Kleiner Israel S.1

Affiliation:

1. From the Department of Physiological Chemistry, New York Homeopathic Medical College and Flower Hospital, New York

Abstract

1. Crystalline urease is not inactivated by trypsin in the absence of a gum. In fact, the presence of trypsin alone in aqueous solutions of urease has an action similar to that of gum, that is, it acts as a "protective colloid" for urease. 2. Crystalline urease is inactivated by trypsin in the presence of a gum. This occurs with great rapidity if purified (crystalline) trypsin is used. 3. If trypsin is added to urease a considerable time before the addition of gum no inactivation of the urease takes place. 4. The suggested explanation is that an inhibitive group of the urease molecule ("antitrypsin") may combine with trypsin to form an irreversible inactive trypsin compound, which cannot attack the urease but has unchanged ureolytic power. This compound cannot be formed if gum is present because the gum has united with a portion of the urease molecule; the tryptic power is thus unimpaired and urease is then digested. This speaks for the protein nature of urease.

Publisher

Rockefeller University Press

Subject

Physiology

Cited by 12 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Polypeptides and Proteins as Inhibitors;Metabolic Inhibitors;1963

2. Amidases;Chemistry and Methods of Enzymes;1953

3. Studies on the Action of Sulfonamides on the Respiration and Growth of Bacteria;Journal of Bacteriology;1945-01

4. The antiproteolytic enzyme ofAscaris lumbricoidesvar.suis;Parasitology;1938-06

5. DOES TRYPSIN INACTIVATE UREASE?;Journal of Biological Chemistry;1937-02

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