THE ADSORPTION OF GELATIN BY COLLODION MEMBRANES

Author:

Palmer Albert H.1

Affiliation:

1. From the Department of Physiology, Yale University, New Haven

Abstract

An experimental study has been made of the adsorption of gelatin from solution at 37°C. by collodion membranes. In the case of membranes of high permeability, very high concentrations of gelatin were required to produce maximum adsorption, and the maximum amounts adsorbed were independent of the pH values of the solutions over the range 3.8 to 4.8. With membranes of low permeability, maximum adsorption was reached at lower gelatin concentrations, and the maximum amounts adsorbed varied with the pH, being lower on either side of the isoelectric point, over the range 3.8 to 6.6. The addition of salt in such experiments raised the maximum amount adsorbed to a value equal to that obtained with solutions at the isoelectric point in the absence of salt. These experiments can be explained by, and seem to lend support to, the theory proposed by Loeb and further developed by Kunitz concerning the effects of pH and salt on the size of gelatin particles in solution.

Publisher

Rockefeller University Press

Subject

Physiology

Cited by 8 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Adsorption Studies of Enzymes and Other Proteins;Advances in Enzymology - and Related Areas of Molecular Biology;2006-11-22

2. Ultrafilter Membranes in Biochemistry;Methods of Biochemical Analysis;2006-10-31

3. Adsorption isotherms on high-performance liquid chromatographic sorbents.;Journal of Chromatography A;1987-10

4. Measurement of invertase activity of cells of Saccharomyces cerevisiae;Analytical Biochemistry;1983-04

5. Adsorption of human serum albumin on precipitated hydroxyapatite;Journal of Colloid and Interface Science;1979-05

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