Turnover of Phosphatidic Acid and Sodium Extrusion from Mammalian Erythrocytes

Author:

Kirschner Leonard B.1,Barker Jennifer1

Affiliation:

1. From the Department of Zoology, Washington State University, Pullman

Abstract

Phosphatidic acid (PA) from swine and beef RBCs was isolated by chromatography on silicic acid columns. It comprised about 1 per cent of the total lipid phosphate in RBCs, but was eluted nearly pure from columns. An uncharacterized inositide accounted for 5 to 10 per cent of the phosphate in the PA-containing fraction. When cells were incubated with HP32O4=, the fraction containing PA became more radioactive than any of the other fractions obtained. However, analysis of the labeled material by paper chromatography showed that most of the P32 was in the inositide, not in PA. With the assumption of kinetic homogeneity for cellular PA, compartmental analysis of the kinetics of tracer incorporation showed that PA turnover is 3 to 4 orders of magnitude too slow to account for sodium extrusion by these cells.

Publisher

Rockefeller University Press

Subject

Physiology

Cited by 12 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Metabolisme phospholipidique des hematies nucleees. Incorporation de [32P] orthophosphate dans les phospholipides erythrocytaires de poulet in vitro;European Journal of Biochemistry;1971-12

2. The Phosphorus Components of Solubilized Erythrocyte Membrane Protein;Canadian Journal of Biochemistry;1971-03-01

3. Di- and triphosphoinositide metabolism in intact swine erythrocytes;Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism;1970-03

4. DI- and triphosphoinositide metabolism in swine erythrocyte membranes;Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism;1970-03

5. ATPase activity in gills of euryhaline fish;Comparative Biochemistry and Physiology;1969-05

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