Two Different Ionic Mechanisms Generating the Spike "Positive" Afterpotential in Molluscan Neurons

Abstract

The ionic bases of the "positive" afterpotential (ap) have been examined in the so-called DInhi neurons of the central nervous system of Cryptomphallus aspersa. In these cells EK has been determined and its value compared with the equilibrium, potential of the ap (Eap). It has been found that in half of the studied cells the EK value is very close to Eap whereas in another half, the difference (EK - Eap) is large and amounts to circa -10 mv. The effects of changes in the concentration gradients of K+, Cl-, and Na+ were assayed in both groups of cells. When the [Ki/[K]o ratio is reduced in both groups of neurons, the ap amplitude and the Eap diminished. In cells displaying a large (EK - Eap), Cl-free Ringer's solution diminished the ap amplitude and Eap, but produced no effect in the neurons with a reduced (EK - Eap). A similar effect was observed if [Cl], was increased by intracellular injection of NaCl. Changes in both [Na]o and [Na]i were ineffective. It is concluded that K+ is the only ion involved in the origin of the ap in the groups of cells with a low value for (EK - Eap). On the contrary, the ap of the neurons presenting large (EK - Eap) is produced by a simultaneous increase in the fluxes of both K+ and Cl-.