Affiliation:
1. From the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts.
Abstract
The proteins in the axoplasm of the squid, Dosidicus gigas, have been resolved electrophoretically into a major fraction including the fibrous protein, and possibly its structural subunits, and a minor fraction including at least two proteins with low sedimentation coefficients. A partially reversible change in the structure of the fibrous protein occurs under the action of 0.4 M salt or high pH. These experiments have been interpreted to indicate that in the intact fiber one, or a few, protofibrils are arranged helically or longitudinally along the fiber axis, and linked by electrostatic bonds. On the dissociation of these bonds the separated protofibrils assume a less extended form and sediment more rapidly than the intact fibers. Some material with a lower sedimentation rate is also released on the dissociation. This fraction may comprise smaller chain fragments. The volume fraction and the approximate refractive index of the fibers have been calculated.
Publisher
Rockefeller University Press
Cited by
49 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. Biochemistry of Neurofilaments and Neurotubules;Ciba Foundation Symposium - Alzheimer's Disease and Related Conditions;2008-05-30
2. ULTRASTRUCTURE OF EXCITABLE MEMBRANES AND THE CRAYFISH MEDIAN-GIANT SYNAPSE;Annals of the New York Academy of Sciences;2006-12-15
3. Neurofilaments;Cellular and Molecular Biology of Intermediate Filaments;1990
4. Biochemistry and Metabolism of the Squid Giant Axon;Current Topics in Membranes and Transport;1984
5. ATP-utilizing systems in the squid axons: A review on the biochemical aspects of ion-transport;Progress in Neurobiology;1982