Affiliation:
1. From the Laboratories of the International Health Division of The Rockefeller Foundation, New York
Abstract
A simple diffusion apparatus has been described in which a layer of solution is allowed to diffuse upward into a layer of solvent. Accurate sampling is performed at various heights and the concentration of the samples is determined.
The method has been illustrated with a determination of the diffusion constant of crystalline catalase, which was found to be 3.1 x 10–7 cm.2/sec. at 4°C. The method should be especially suited to the study of biological substances endowed with specific activity and which cannot be obtained in pure solution.
Publisher
Rockefeller University Press
Cited by
11 articles.
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