TRACER STUDIES OF NITROGEN ASSIMILATION IN YEAST

Abstract

By using N15 as a tracer the assimilation of ammonia by the yeast, Torulopsis utilis, has been studied. It has been shown that: 1. There was no measurable incorporation of N in the protein or polynucleotide purine of carbon-starved yeast. 2. When ammonia is added to nitrogen-starved yeast there is a long lag period before division begins during which the yeast rapidly synthesizes protein, this process being accompanied by a turnover of polynucleotide purine. There was no significant dilution of the N15H4+ of the medium by ordinary NH4+. 3. When yeast containing N15 is allowed to divide and grow in ordinary ammonia, the total amount of N15 in the yeast remains constant. The dicarboxylic amino acids are most diluted, while arginine and nucleic acid guanine are not diluted at all.