Selection and characterization of DNAzymes with synthetically appended functionalities: A case of a synthetic RNAsea mimic

Author:

Ting R.1,Lermer Leonard1,Thomas J.1,Roupioz Y.1,Perrin D. M.1

Affiliation:

1. 1Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T-1Z1, Canada

Abstract

We have been interested in merging synthetic nucleotide chemistry with combinatorial selection of DNAzymes to deliver a more complete (and complex) chemical complement to the catalytic repertoire of nucleic acids. Thus we ask, what do modified dNTPs really bring to nucleic acids in terms of an increased repertoire? In asking this question, we have looked first at conditions, and more recently for reaction classes where nucleic acids are found to be catalytically inefficient, deficient, or at least to date, seemingly incapable of certain functions. A case of this is M2+-independent ribophosphodiester hydrolysis at physiological pH and low ionic strength where nucleic acids exhibit especially low rate constants for self-cleavage and seem to be incapable of turnover.

Publisher

Walter de Gruyter GmbH

Subject

General Chemical Engineering,General Chemistry

Reference3 articles.

1. Pure;Benner;Appl Chem,1998

2. Pure and;Battersby;Am Chem Soc Applied Chemistry Proc Natl Acad Sci USA,1999

3. Pure and DNAzymes with synthetically appended functionalities;Curtis;RNA Applied Chemistry RNA,2001

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