A method development for sertraline detection in the blood

Abstract

Aim – to develop a method for isolation and determination of sertraline in biological fluids. Material and methods. For the study we used "Zoloft" drug ("Pfizer", USA). The study equipment and substances: liquid chromatograph Shimadzu LC-20 Prominence (Japan) with detector SPD-M20A, gas chromatograph Agilent Technologies (USA) 7890 A / 5977 MSD, (program MassHunter GC-MS ), waters vacuum unit and cartridges for solid-phase extraction Oasis HLB; papain enzymes (MedFloran, Russia); trypsin, chymotrypsin, hyaluronidase (Lidaza) (Samson-Med LLC, Russia); test strips from different manufacturers under trade names: "Be sure", "NarcoCHEC", "FACTOR-MED". Urine samples were obtained after administration of the drug to laboratory animals – guinea pigs. The base of sertraline from a solution of its salt was isolated by liquid-liquid extraction (LLE) with organic solvents and their mixtures at pH = 10, 11 and 12 (pKa sertraline 9.48). Obtaining data on the effectiveness of blood sample preparation methods (liquid-liquid extraction, solid-phase extraction and enzymatic hydrolysis) for sertraline was carried out on enriched model samples of donor blood according to the method proposed by Cheger. Results. Cross-positive urine test results were obtained with immunochromatographic test strips for derivatives of e 1,4-benzodiazepine and synthetic cannabimimetics ("spice"). The study with 1 mg/ml solution of sertraline gave a negative result. Desmethylsertraline, a major metabolite, was detected in the urine, a peak with a retention time of about 12.75 min, the peak of the original substance during the synthesis of sertraline – sertralinimine and the peak of the native sertraline with a retention time of about 12.65 min. A method for detecting sertraline by HPLC has been developed. Conclusion. The use of the enzymatic blood hydrolysis with trypsin allows for increasing the degree of extraction by 1.5 times when compared with liquid-liquid extraction with chloroform, and by 2 times when compared with solid-phase extraction.