THE ROLE OF HSA-MIR-451A IN THE GROWTH AND PROGRESSION OF LUMINAL BREAST CANCER

Abstract

Numerous genetic and biological processes have been linked to the function of microRNAs which regulate gene expression by targeting messenger RNA mRNA. MicroRNAs play a role in the development of disease and the embryology of mammals. To further understand its function in the oncogenic process, the expression of the microRNA profile in cancer has been investigated.Despite being referred to as a noteworthy microRNA in cancer, it is unknown whether hsa-miR-451a plays a part in the in vitro progression of the luminal A and luminal B subtypes of breast cancer. Activity of hsa-miR-451a was examined when it was transfected and expressed in breast cancer cell lines known as luminal A and luminal B, in terms of the normal stages of cancer progression in vitro, such as proliferation, survival, migration, and invasion. We also thoroughly investigated target score-based bioinformatics algorithms for predicting microRNA-mRNA interactions. The progression (proliferation, migration, invasion) of luminal A and B breast cancer cell lines in vitro was markedly slowed by the expression of exogenous hsa-miR-451a. Based on bioinformatics analysis, we found a powerful interaction between hsa-miR-451a and the genes for CAB39, AKT1, and BRAF. In silico and experimental results confirmed a detailed gene network that boosts the activation of signaling pathways required for cancer progression and under expression of hsa-miR-451a it was decreased.Absence of hsa-miR-451a in breast cancer tissue and its correlation with prognosis and tumor behavior provides a significant contribution that supports current contexts such as liquid biopsies and precision oncology based on bioinformatics tools and data mining.