Quantitative Conductometric Determination of Sitagliptin, Linagliptin, Vildagliptin and Alogliptin by Applying the Concept of Drug-Metal Ion Interaction

Abstract

Cost of analysis and length of analytical procedure are among the most concerning factors in drug analysis. As conductometric analysis has been considered to be relatively inexpensive analytical technique offering fast analysis of drugs, in this study our aim was to develop a rapid and cost-effective method for quantitative determination of sitagliptin, linagliptin, vildagliptin and alogliptin in bulk and dosage forms. The test drugs were allowed to complex with metal ion (Cu2+) in the titration cell, which resulted in the change of conductance of the solution. The corrected conductance was calculated and graph was plotted between corrected conductance and the volume of the analyte solution added. The point of maximum change in the corrected conductance was considered as end point of the titration. The method was found to be linear in the concentration range of 1.0 – 1.4 mM for all analytes with good correlation coefficient (R2 ˃ 0.999). The %RSD of the corrected conductance values were in the range of 0.046-1.837, while the recovery of analytes were within 100 ± 2%, indicating that the method was precise and accurate. The specificity of the method was demonstrated by no interference from blank and placebo. The method was successfully applied for quantitative analysis of all the drugs in the dosage forms. The current method has a major advantage that it provided easy, fast and economical analysis of sitagliptin, linagliptin, vildagliptin and alogliptin in bulk drugs and formulations using conductivity meter. KEYWORDS: