Visible Spectrophotometric Determination of Gemigliptin using Charge Transfer Complex-Reference-Cited by-同舟云学术

Visible Spectrophotometric Determination of Gemigliptin using Charge Transfer Complex

Published:2019-04-18 Issue:2 Volume:35 Page:694-699
ISSN:0970-020X
Container-title:Oriental Journal of Chemistry
language:
Short-container-title:Orient. J. Chem

Author:

Gorumutchu Giri Prasad¹,Nadh Ratnakaram Venkata²^ORCID,V. N. V Kishore³

Affiliation:

1. Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, India.

2. GITAM University – Bengaluru, Karnataka-562163, India.

3. http://orcid.org/0000-0003-0925-1132

Abstract

A visible spectrophotometric method was developed and validated for the determination of gemigliptin present in bulk drug and tablet formulation. It involves an indirect method of charge transfer complex formation in presence of NBS, metol and suphanilic acid. Gemigliptin was subjected to oxidation with excess amount of oxidant (NBS) and the unconsumed NBS oxidizes metol to give p-N-methylbenzoquinone monoamine (PNMM) which in turn forms a charge transfer complex with sulphanilic acid. Then validated the above developed method as per the current ICH guidelines. An excellent correlation coefficient (> 0.999) was found for the obtained regression equation (y = –0.0302x + 0.928) in the range of 2.0–30.0 μg mL-1. The method was found to be simple and rapid because it does not involve any solvent extraction. The recovery levels of the drug were in the range 99.92 – 100.08.

Publisher

Oriental Scientific Publishing Company

Subject

Drug Discovery,Environmental Chemistry,Biochemistry,General Chemistry

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