Affiliation:
1. A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia
Abstract
This paper considers the combination of several immunochemical interactions for multiplex immunochromatographic analysis. It was shown that varying the composition of the gold nanoparticles–antibodies conjugate, its concentration and concentration of the hapten–protein conjugate changes both the maximal binding in the test zone and the range of the determined concentrations of the target analyte. Thus, it becomes possible to compensate for differences in the interaction times for the reagents of different specificities that arise when they are combined on one test strip. These regularities were demonstrated for multiplex analysis of three antibiotics. The developed assay has limits of detection of 10, 500, and 10 ng/mL for chloramphenicol, streptomycin, and ampicillin, respectively. Its working ranges for these three analytes are 0.5–6, 16–250, and 0.4–5 ng/mL. The analysis time is 15 min; the accuracy of the photometric determination of antibiotics in the working ranges is up to 6%.
Funder
Ministry of Education and Science of the Russian Federation
Publisher
Oriental Scientific Publishing Company
Subject
Drug Discovery,Environmental Chemistry,Biochemistry,General Chemistry
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