Docking of Some Bioactive 2-Chloro-N, N-Diphenylacetamide Derivatives on Cyclo-Oxygenase Enzyme and In-Vivo Analgesic Activity Evaluation

Abstract

The present work was aimed to synthesize some novel 2-chloro-N,N-diphenylacetamide derivatives conjugated with various benzaldehydes in multistep synthesis and further to undergo the process of docking on COX-1 and COX-2 enzymes. All the targeted synthesized compounds were subjected to evaluation of analgesic activity using hot plate model. Sophisticate analytical techniques via infrared spectroscopy, NMR, Mass spectroscopy and elemental analysis were employed to characterize the newly synthesized compounds. All the synthesized derivatives were evaluated for their analgesic activity. The synthesized compound AKM-2 (N-(4-(diphenylamino) thiazol-2-yl)-2-((4-((3methylbenzylidene)amino)phenyl)amino)acetamide) exhibited significant analgesic response when comparison was made with diclofenac sodium as standard drug. Molecular docking study was used to discover the possibility of binding site and binding strength of new derivatives of acetamide. The present study offers insight for compound AKM-2, as a new lead compound as analgesic agent because of significant docking and similar amino acid residue. The biological activity performed by in-vivo method confirmed the same.