Screening of Stem Extracts of Andrographis echioides for Anti-Inflammatory, Anti-Arthritic, Anti-Microbial, and AntiCancer Activity against MCF-7 Human Breast Cancer Cell Lines

Abstract

Nowadays breast cancer in rheumatoid arthritis patients is the most controversial one. Rheumatoid arthritis is an inflammatory autoimmune disorder. RA may induce the order disease like cancer especially breast cancer in most women. Breast cancer starts when the breast cell begins to grow out of control. Inflammatory, anticancer drugs may show severe side effects. To overcome these herbal plants are used to make drastic changes in medical treatments. The main objective of this paper is assessment of Anti-inflammatory, anti-arthritic, anti-microbial and anti-cancer activity against the Michigan cancer foundation (MCF-7) human breast cancer cell line using the extracts Andrographis echioides. Anti-inflammatory and anti-arthritic activity show maximum inhibition of albumin denaturation, anti-proteinase, hypo-tonicity induced hemolysis, anti-lipoxygenase activity with standard drugs such as aspirin, diclofenac sodium and indomethacin at the concentration of 100µg/ml under in vitro condition. Thus the potential inhibition of protein (egg albumin) denaturation (85.80%) and for the human red blood cell (HRBC) membrane stabilization model 85.50% was observed at the concentration of 100µg/ml. The antioxidant and cytotoxicity activity of Andrographis echioides showed better inhibition in a dose-dependent manner against MCF-7 human breast cancer cell lines. The antimicrobial activity of Andrographis echioides showed maximum inhibition of E.coli (11mm), Enterococcus aerogenes and Staphylococcus aureus (10mm), and Candida albicans (11mm) at 100µg/ml concentration. The innovation of the present study revealed that the stem of Andrographis echioides have potential source for natural anti-arthritis, cytotoxicity, anti-microbial, antioxidant activity, anti-inflammatory and anticancer activities.