Anethum Graveolens Leaves Extract Accelerate Wound Healing In vitro and In vivo

Abstract

Wound healing is a curative process that starts with trauma and finishes with scar formation. Various plant extracts have been used for the treatment and controlling of wounds. In this study Anethum graveolens has been used to accelerate in vivo excision model of wound healing on Sprague Dawley rats and to proliferate the in vitro cell viability model using skin fibroblast cell line through the scratch assay. Results confirm that this plant extract decreases the wound area and increases itswound size reduction percentage, hydroxyproline and nitric oxide levels of the plant extract treated groups were near to the normal control group that indicated effective healing process. On the other hand, in vitro cytotoxicity results should that Anethum graveolens plant extract was safe on skin fibroblast cell lines and induced the normal proliferation and growth of these cells. The migration rate to heal the in vitro wound gaps was 89.1% which indicates a perfect wound size reduction. In conclusion, the results proved that the topical application of Anethum graveolens plant extract quicken the wound healing process.