The Morphological Effects of Methandienone on Sperm Head in Male Mice

Abstract

The objective of the present study is to detect the effects of methandienone on sperm head morphology of male (Mus musculus) mice. For this purpose 55 adult male mice were used and divided into five groups: both of the control -ve and control +ve (CFA) have consisted of 5 mice, and the other three treated groups consisted of 15 mice injected orally with three doses (low, medium and high) (0.125, 0.25, 0.5) mgkg body weight methandienone, with periods (7, 21, 35) days. At the end of the treatment periods morphological abnormalities of sperm were examed. The results showed the presence of abnormal change in the form of the sperms head morphology, the effect of methandienone was dose and time dependents. The significant abnormal shapes of sperms head were observed; they were swelling head, head acrosome loss, apical hook, hammerhead, acrosome defective and other abnormalities. From these results, we can conclude Methadinone has the potential to increase the rate of deformity of head sperm morphology when increasing dose and duration of the drug, indicating that may be a genetic damage happened affects the stages of sperm formation and this needs further studies and in spite of methandienone medical uses and advantages, dose and period of treatment must be determined by a doctor.