Evaluation of the Analgesic Activity of Ethanolic Extract of Manilkara Zapota Seeds in Experimental Animal Model

Abstract

Introduction: Non-Steroidal Anti-Inflammatory Drugs and opioids use remain the mainstay for pain treatment. However, both groups of drugs are well known for their common and severe side effects. Medicinal plants could be the better option to overcome these side effects. The anti-inflammatory activity of seeds of Manilkara zapota has already been demonstrated in experimental animals. As pain always accompanies inflammation, we have set forward a study to discover the analgesic activity of the ethanolic extract of Manilkara Zapota in animal models using Eddy's hot plate method. Aims and objectives: To explore the analgesic activity of ethanolic extract of Manilkara zapota seeds using Eddy's hot plate method. To compare the effect of ethanolic extract of Manilkara zapota seeds with the commonly used analgesic drug aspirin. Material and Methods: This study was conducted by using Eddy's hot plate method. A total of 12 Adult Wistar albino rats were grouped into three groups of 4 animals in each group. These groups were Group I: Control (normal saline), Group II: Standard (Aspirin 25mg per kg) Group III: Ethanolic extract of Manilkara zapota (200mg per kg). The reaction time was recorded at 0, 30, 60 & 90 minutes after injecting normal saline, standard drug, and extract. Result: The increase in mean reaction time of the extract was statistically significant (p< 0.0001) at 30, 60, and 90 min compared to that of the control. There was less increase in the reaction time of extract treated group compared to that of the aspirin-treated group at 30 min & 60 min points. Conclusion: The analgesic activity of the extract was significantly more than that of the control group. Peak analgesic activity occurred at 60 and 90 minutes.