A Pilot Study on Aetiology of Acute Lower Respiratory Tract Infections Among Children Hospitalized of Respiratory Illness at a Rural Hospital in South Coastal Karnataka

Abstract

Objective: Aetiological diagnosis can significantly impact the clinical management and outcome of acute lower respiratory tract infections (LRTI) in children. There is a paucity of data on etiological agents of acute LRTI among children in Karnataka, especially in Udupi district. Present study provides an insight into the pathogens associated with acute LRTI among children in Udupi district of south coastal Karnataka. Methods: A cross sectional study was performed at a rural hospital in south coastal Karnataka, A total of 50 children clinically diagnosed for acute LRTI and admitted in paediatric ward were enrolled for the study. Nasopharyngeal/throat swab specimens were collected, and nucleic acid was extracted, and Multiplex real-time PCR was performed for detection of bacterial and viral aetiology. Results: S. pneumoniae was detected in 16% (8/50), followed by ‎ Respiratory syncytial virus (RSV) 14% (7/50), H. influenzae 8 % (4/50) and M. pneumoniae 2% (1/50). Mixed infection was detected in 28% (14/50) of children. S. pneumoniae and H. influenzae was the most prevalent co-infection and was detected in 10% (5/50) followed by H. Influenzae and RSV (4%, 2/50) co-infection. Conclusion: S. pneumoniae and RSV were the most predominant bacterial and viral pathogens respectively associated with LRTIs among paediatric population in present study. Further we found very high number of cases with mixed infections which signifies the urgent need of much elaborate studies for elucidating the clinical significance of these infections as well as for better understanding of epidemiology of LRTI among children in this region.