Melittin-Induced Cell Death Through p53 and 8-OHdG in Breast Cell Cancer MCF-7-Reference-Cited by-同舟云学术

Melittin-Induced Cell Death Through p53 and 8-OHdG in Breast Cell Cancer MCF-7

Published:2022-06-30 Issue:2 Volume:15 Page:979-983
ISSN:2456-2610
Container-title:Biomedical and Pharmacology Journal
language:en
Short-container-title:Biomed. Pharmacol. J.

Author:

Plasay Makkasau¹^ORCID,Natzir Rosdiana²^ORCID,Cangara Muhammad Husni³^ORCID,Hardjo Marhaen²,Syahrijuita Syahrijuita²^ORCID,Soraya Gita Vita⁴^ORCID

Affiliation:

1. 1Master of Biomedical Study Program, Post Graduate School, Hasanuddin University, Makassar, South Sulawesi, Indonesia

2. 3Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia

3. 4Department of Anatomical Pathology, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia

4. https://orcid.org/0000-0002-2656-4176

Abstract

Melittin, one of the cytolytic peptides derived from bee venom, is a broad-spectrum efficacy candidate as an antibacterial, antifungal, and antitumor agent. This study demonstrates the cytotoxic effect of melittin isolated from Apis mellifera through the induction of p53 and 8-OHdG. The antiproliferative effect was evaluated against breast cell cancer MCF-7 via MTT assay, while the molecular mechanism of melittin on MCF-7 was assayed by p53 and 8-OHdG ELISA. With an IC50 value of 5.86 µg/mL ((very toxic)), the cytotoxic impact inhibits MCF-7 cell proliferation in a dose-dependent manner. Significant (p < 0.05) elevations in the level of p53 and 8-OHdG were evident in the IC50-treated cells compared to control. In conclusion, melittin may have considerable potential as a novel natural product-based for breast cancer.

Publisher

Oriental Scientific Publishing Company

Subject

Pharmacology

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