Simultaneous Quantification of Vitamin A and E in Pharmaceutical Formulations and fruit Samples Using Fe(II)-1-Nitroso-2-Napthol Complex

Abstract

A rapid and sensitive spectrophotometric method for the determination of vitamin A and vitamin E using Fe(II)-1-nitroso-2-napthol detection system is reported. The in situ reduced Fe(II) formed green coloured complex with 1-nitroso-2-napthol which gives an absorption maxima at 708nm. Beer’s law was obeyed in the range 4.0-14.0μg/mL for vitamin A and 4.0-24.0μg/mL for vitamin E with relative standard deviation 1.7% for vitamin A and 2.2% for vitamin E. The molar absorptivity was found to be 1.3×104 L mol-1 cm-1 for vitamin A and 2.5×104 L mol-1 cm-1 for vitamin E with the co-relation coefficients 0.996 and 0.935 for vitamin A and vitamin E respectively. Optimization of various factors was done to get the maximum absorption value. The proposed method is utilized satisfactorily for pharmaceuticals, milk sample,fruit sample and different liquid samples of vitamin A and vitamin E by pre treatment of samples.