In Vivo Analgesic Activity of Omega-3 on Mice Induced Peripheral Pain

Abstract

Fish oil which contains omega 3 with the most types of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) has the effect of pharmacology as anti-inflammatory, antioxidant and is good for heart disease where probably it can be used beneficially as an analgesic. Analgesics have several side effects including gastric ulcer, bleeding, even Steven Johson’s Syndrome, have been attributed to their use. This research aims to determine the analgesic effect of fish oil on male white mice induced to produced peripheral pain. We used acetic acid-induced male BALB/c white mice to investigate analgesic effect fish oil in vivo by Writhing method. From this research, fish oil decreased writhings number in mice dose 5, 10, 20 and 30 mg/kg compared to negative control (P<0.001). The percentage inhibition of writhing at the dose 30 mg/kg is the hignest among other dose (42.64%), while in the group positive control (acetylsalysilic acid 100 mg/kg) is 68.26%. We concluded that fish oil has analgesic effect which reduced writhings in male mice induced by acetic acid to produce peripheral pain.