Extracorporeal Circuit Device for Camel Antibodies Production from Blood using Magnetic Nanoparticles

Abstract

ABSTRACT: The present work describes the use of nanotechnology in the direct purification of antibodies from life camels. The new method relies on the use of a simple extracorporeal device coupled with affinity purification to antigen functionalized magnetic nanoparticles that is applied continuously in extracorporeal circuit. The device was connected to camel blood stream by HLS cannulae veno-venous to allow the blood stream path through the device with the flow rate of ~ 10 ml/min. The new method requires no sacrificing the animal for the recovery of antibodies. Antigen functionalized magnetic nanoparticles are injected into a blood flow where they capture the targeted antibodies by affinity binding. Subsequent removal of the antibody-loaded nanomagnets from the bloodstream takes place by continuous magnetic separation allowing recirculation of the blood for continuous production of more antibodies. For the demonstration of the possible use of this new method in purifying the antibodies from immunized camels, lysozyme was used as the antigen for immunization and magnetic nanoparticles functionalization. Immunization of camels was performed for a period of eight-week with gradual increase of antigen amount up to 5 or 1 mg antigen. The device was validated via Sandwich ELISA and results indicated the efficacy of the new device in the continuous recovery of camelid antibodies without the need to sacrifice the animal and the need to use antigen at no more than 1 mg for successful antibodies production using the new device at a large scale.