Phytochemical and Antimicrobial Analysis of Root Extract of Aspargus Adscendens

Abstract

The aim of this study was to characterize the phytochemicals and to investigate the antimicrobial efficacy of the shade dried roots of the Asparagus adscendens. The powder roots were extracted with chloroform and after removal of solvent under reduced pressure was subjected for chromatographic separation. After column chromatography and purification five compounds i.e .hexacosanyltriacontanoate, hexatriacontanone, 2,3,5,7-tetramethoxydi hydrophenanthrene, -sitosterol and stigmasterol were characterized with the help of physical and spectral analysis(IR, 1HNMR, 13CNMR and mass). The crude extract was tested against the selected bacteria and fungi using disc diffusion method. The root extract exhibited significant antibacterial activity with maximum efficacy against E. coli (activity index 0.82 at 1000 µg/disc and 0.73 at 500 µg/disc).