Effects of Amnion Lyophilization Sterile Radiation on Vascular Endothelial Growth Factor and Cluster of Differentiation 34 in Nonunion Fractures-Reference-Cited by-同舟云学术

Effects of Amnion Lyophilization Sterile Radiation on Vascular Endothelial Growth Factor and Cluster of Differentiation 34 in Nonunion Fractures

Published:2022-06-30 Issue:2 Volume:15 Page:1053-1057
ISSN:2456-2610
Container-title:Biomedical and Pharmacology Journal
language:en
Short-container-title:Biomed. Pharmacol. J.

Author:

Fauzi Ahmad¹,Alvarino Alvarino²^ORCID,Yanwirasti Yanwirasti²^ORCID,Sahputra Roni Eka²^ORCID

Affiliation:

1. 1Student of Doctoral Program in Biomedical Science, Universitas Andalas, Padang, Indonesia.

2. 2Faculty of Medicine, Universitas Andalas, Padang, Indonesia.

Abstract

One of the causes of nonunion is inadequate vascularization due to severe injury mechanisms that cause defective bone healing factors. Therefore, in non-union fractures, a trigger is needed for the growth factors to work properly. This study aimed to determine the effects of ALSR on VEGF and CD-34 in non-union fractures. This study used an experimental post-test only control group design that involved white rats of the Sprague-Dawley strain. The VEGF expression was assessed by anti-VEGF staining, which was done by cutting paraffin blocks into a thickness of 5 μm, then the blocks were deparaffinized in xylol and rehydrated with alcohol. The results of the analysis are displayed with their mean ranks between control groups and treatment groups. It was found that CD-34 expression was high in the ALSR group. This shows the occurrence of angiogenesis and the regeneration in the case of non-union fractures in this study.

Publisher

Oriental Scientific Publishing Company

Subject

Pharmacology

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