Acacia Gerrardii Leaf Extracts Inhibit Genetic Diversity Induced by Streptozotocin in Male Rats

Abstract

This study investigated the prevention effect of Acacia gerrardii leaf extracts on genetic variation in male rats exposed to streptozotocin-induced diabetes. Male rats (n=70) were treated with A. gerrardii leaf extract using concentrations ranging from 100 to 500 mg/kg body weight/day for four weeks against streptozotocin-induced possibly genetic variation. Blood samples and pancreatic tissues were collected at the end of the experiment. Ten Inter-Simple Sequence Repeat primers and ten Start Codon Targeted Polymorphism primers were used to detect genetic variations between the different groups. The biochemical results indicated a reduction in serum glucose levels, especially at doses of 400 and 500 mg/kg body weight. Molecular analysis showed that Inter-Simple Sequence Repeat primers and SCoT detected 119 and 97 amplicons, with 0.43% and 0.55% polymorphic respectively. Based on the previous markers analyzed, cluster analysis revealed that genetic variation was high in the pancreatic tissues of streptozotocin-treated rats in the groups treated with 0 (C+) > 100 (T1) > 200 (T2) > 300 (T3) mg/kg body weight/day. However, low genetic variation was observed in the pancreatic tissues of streptozotocin-treated rats in the groups treated with 400 (T4) > 500 (T5) mg/kg body weight/day. These results indicate that the A. gerrardii leaf extracts are rich in antioxidants preventing genetic variation in the pancreatic tissues. Therefore, A. gerrardii leaf extracts could be recommended as good source of dietary antioxidant