Effect of Carbon Source on Biomass and Flavonoid Content of Gynuraprocumbens (Lour.) Merr Adventitious Root in Liquid Culture

Abstract

Gynura procumbens (Lour) Merr is a medicinal plant that has been widely used as an ingredient of herbal medicine. G. procumbens is known to contains secondary metabolite compounds namely flavonoids. The aim of this study was to investigate the effect of carbon sources on biomass and flavonoid content of G. procumbens adventitious root culture grown in agitated liquid medium. Adventitious roots were induced from leaf explants on Murashige and Skoog (MS) medium supplemented with 3% sucrose and 8 g/Lagar. G. procumbens adventitious root cultures were performed in MS liquid medium containing different carbon source of 3% sucrose, 5% sucrose, 3% glucose and 4% fructose, and supplemented with5 mg/L IBA. Two grams of adventitious roots as the initial inoculum were cultured in1000 mL Erlenmeyer flask containing 250 mL MS medium and agitated at 70 rpm in dark conditions for 28 days.The results showed that medium with the addition of 5% sucrose produced the highest fresh weight (10.23 ± 0.86 g) followed by 3% sucrose (8.39 ± 0.60 g), while the addition of 3% glucose (3.35 ± 0.11 g) and 4% fructose (3.68 ± 1.13 g) produced adventitious root biomass lower compared to the addition of sucrose.The production of G. procumbens adventitious roots biomass increased 5-fold of the initial inoculum. The highest flavonoid content was obtained in MS medium supplemented with 3% glucose with the content of kaempferol and quercetin reached 25.44g/L per 1gdry weight and 7.08 g/L per gdry weight, respectively.