The Microanatomical Study of Effect of Alkaloid Isolate of Dioscorea Bulbifera Bulbis on Cyclophosphamide Induced Testicular Damage in Adult Male Wistar Rats

Abstract

Cyclophosphamide is a synthetized drug and alkylating agent used for treating cancer. In this research, cyclophosphamide's effect on the testicles of adult male Wistar rats will be assessed, as well as the impact of alkaloid extract on the induced damage. Twenty-four healthy male wistar rats weighing between 132g to 168g of the same species of rattus Norvegicus were used. Rats were randomly assigned to 4 groups, A through D (n=7 in each). Group A was given distilled water. Group B were administered with Cyclophosphamide 150mg/ kg intra-peritoneal route for seven days. Group C was administered with Cyclophosphamide 150 mg/kg and 50 mg / kg of Alkaloid concomitantly for a week and Group D was administered Alkaloid for 7 days and then Cyclophosphamide of 150 mg /kg for 7 days. There was a significant difference in weight change, according to one way ANOVA result (F = 175.9; p < 0.001), testosterone activity (F = 7.019; p = 0.0125), follicle stimulating hormone activity (F = 13.27; p = 0.0018), sperm motility (F = 11.95; p = 0.0025) in group B (cyclophosphamide only) as compared to control and across all experimental groups. Cyclophosphamide administration was observed to have a negative effect on the testicular histology and immunohistochemical results. The administration of alkaloids both concomitantly and as a pre-treatment helped to counteract the effect of the drug. In conclusion, the administration of cyclophosphamide should be strictly monitored and given in low doses alongside alkaloid to prevent toxic effect.