Hibiscus Sabdariffa L. Nanoparticles Offer a Preventive Potential Against Experimental Ehrlich Solid Carcinoma

Abstract

Hibiscus sabdariffa L. has been widely cultivated in tropical areas, usually used in treatment of many disorders. Thus, in our study we aimed to evaluate the effect of dairy desserts supplemented with nanoform of Hibiscus sabdariffa L. extract (NHSE) against Ehrlich solid carcinoma (ESC) in mice. The NHSE was prepared by soaked the fine powder of plant in 90% ethanol by cold extraction. NHSE was evaluated using dynamic light scattering (DLS) and transmission electron microscope (TEM), then the prepared NHSE was added to dairy desserts using different concentrations. Sixty female albino mice were used and divided into six groups. After the end of the experimental period, blood was withdrawn; Serum was separated for determination of malondialdehyde (MDA), super oxidedismutase (SOD), catalase (CAT), tumor necrosis factor- α (TNF-α), matrix metalloproteinases-9 (MMP-9) and B-cell lymphoma-2 (Bcl-2). Serum homocystein (Hcy) level was estimated by high performance liquid chromatography (HPLC). Mice inoculated intramuscularly with Ehrlich cell line showed statistically marked increase in serum levels of MDA, TNF-α, MMP-9 and Hcy accompanied by marked decrease in SOD and CAT activities and Bcl-2 levels compared to the control group. Treatments with NHSE markedly trigger activity of anti-oxidant, attenuated the inflammatory response, reduced levels of Hcy and stimulated the apoptosis of tumor cells. Based on that, dairy desserts containing NHSE showed effective role in prohibiting the releasing of reactive oxygen species, ameliorating the immune response, and preventing tumor progression.