Effect of Burdock Root Oil on Oxidative Stress Induced by Isolated and Combined Use of Gamma Radiation and Hexavalent Chromium

Abstract

They studied the effect of the herbal medicine "Burdock root oil" on oxidative damage to liver, kidney and blood tissues. The experiment was performed on 50 non-linear white male rats weighing 180-220 g, divided into 5 groups. The first group - control; animals of groups II and III were subjected to fractional gamma irradiation for five days (0.6 G/day; dose rate 1 Gr/min (60Co)). The total dose was 3 Gr. Animals of the fourth and fifth groups were exposed to a combination of gradiation (as in groups I and III) and potassium dichromate (Cr+6). Potassium dichromate was administered intraperitoneally daily at a dose of 2.8 mg/kg of body weight (0.1LD50) for 5 days (0.5LD50). Rats of groups 3 and 5 received Burdock root oil at a dose of 2.5 ml/kg of body weight intragastrically for 14 days prior to the experimental exposure. Fractional exposure, combined exposure g-radiation, gamma radiation and Cr+6 led to an increase in malondialdehyde and diene conjugates in blood plasma, liver and kidney tissues. Under g-irradiation, the activity of superoxide dismutase enzymes (SOD) and catalase (CAT) in red blood cells compensation increased significantly against the background of a decrease in the level of SH-groups in blood plasma. In liver and kidney tissues, all studied enzymes and reduced glutathione (GSH) levels decreased. Under the conditions of combined exposure g-radiation and potassium dichromate - all the studied indicators of antioxidant protection decreased. The introduction of Burdock root oil before isolated and combined exposure provided significant antioxidant protection in the studied tissues. Conclusion: it can be assumed, that the "Burdock root oil" it is a potential drug that can be used as a radiation protector, in conditions of combined influence of a physical and chemical agent-a detoxifier. In our opinion, the antioxidant potential of the herbal medicine justifies the continuation of further research in clinical practice.