An Analysis of Khurtsiin Deed-6 Compound’s Effect on IL-1β Expression in Nitroglycerin Induced Migraine and Alcohol Exposed Neurodegeneration Model in Rat-Reference-Cited by-同舟云学术

An Analysis of Khurtsiin Deed-6 Compound’s Effect on IL-1β Expression in Nitroglycerin Induced Migraine and Alcohol Exposed Neurodegeneration Model in Rat

Published:2022-09-29 Issue:3 Volume:15 Page:1687-1693
ISSN:2456-2610
Container-title:Biomedical and Pharmacology Journal
language:en
Short-container-title:Biomed. Pharmacol. J.

Author:

Gombodorj Jargalsaikhan¹^ORCID,Bayarsaikhan Enkhjargal²^ORCID,Chimedtseren Chimedragchaa³^ORCID,Baatartsogt Uuganbayar³,Gansukh Baigali³,Surenjid Seesregdorj¹

Affiliation:

1. 1International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.

2. 3National cancer center of Mongolia, Ulaanbaatar, Mongolia.

3. 2Institute of Traditional Medicine and Technology, Ulaanbaatar, Mongolia.

Abstract

Objectives: To investigate the anti-inflammatory activity of Khurtsiin deed-6 in migraine and neurodegeneration rat models. Methods: This study uses nitroglycerin induced migraine was model and alcohol exposed neurodegeneration model in Wistar rats to evaluate anti-inflammatory effect of Khurtsiin deed-6 at the 3 different doses of 50, 100and 150 mg/kg, orally. After anesthesia, the brains were removed, then trigeminal nucleus caudal is and hippocampus region isolated from fresh brain. And followed by protein and RNA extraction. Interleukin-1β expression was analyzed by real time polymerase chain reaction. Results: Nitroglycerin induced rat migraine model had increased Interleukin-1β expression in brain TNC area (p<0.001). It has been decreased dramatically after treatment of Khurtsiin deed-6 by doses of 50mg/kg, 100mg/kg and 150mg/kg treatment in brain trigeminal nucleus caudal is region (p<0.01) comparing ibuprofen treated group. Moreover alcohol exposed neurodegeneration rat model had observed increased Interleukin-1β expression in brain Hp area (p<0.001). But Khurtsiin deed-6 doses of 50mg/kg, 100mg/kg and 150mg/kg treatment reduced neuroinflammatory cytokines IL1β expression in hippocampus region (p<0.01, p<0.001) of alcoholic rat models comparing pyridoxamine administrated animals. Conclusion: The present finding indicates that Khurtsiin deed-6shows anti-inflammatory activity decreasing the level of Interleukin -1β cytokine in nitroglycerin induced migraine and alcohol exposed neurodegeneration rat models

Publisher

Oriental Scientific Publishing Company

Subject

Pharmacology

Reference21 articles.

1. 1. G. Tsagaankhuu, Neurology, 2th ed. Ulaanbaatar, 2011.

2. 2. Luvsanchoimbol, “‘Jedun ninor’ Traditional Medical source book,” “Mongol Printing” printing house, Ulaanbaatar, p. 16, 1993.

3. 3. D. B. C. Ch, A reference book of traditional drug and medicinal materials, 2th ed. Ulaanbaatar, Mongolia: Jicom press, 2015.

4. 4. C. C. Myadagbadam U, Purevsuren S, Norovnyam R, “Results of the quality and safety parameters of the Khurtsiin deed-6 traditional medicinr.” Ulaanbaatar, Mongolia, 2021.

5. 5. D. B. Reddy and P. Reddanna, “Chebulagic acid (CA) attenuates LPS-induced inflammation by suppressing NF-κB and MAPK activation in RAW 264.7 macrophages,” Biochem. Biophys. Res. Commun., vol. 381, no. 1, pp. 112–117, 2009, doi: 10.1016/j.bbrc.2009.02.022.