Analytical Method Development and Validation of Quetiapine Fumarate in API and Dosage form by Using RP–HPLC

Author:

Lingareddygari* Siva Sanker Reddy1ORCID,Ravikrindhi Nageswara Rao1

Affiliation:

1. Department of Pharmaceutical Analysis, Santhiram College of Pharmacy, Panyam, Nandyal, Andhra Pradesh, India.

Abstract

RP-HPLC method developed is a simple, precise and functional technique for the calculation of amount of Quetiapine fumarate from marketed tablets and bulk form. The RP-HPLC analysis was carried out on Hyper chrome ODS-BP 5µm column (4.6mm × 200mm) using a mobile phase 0.1% Orthophoshoric acid and Acetonitrile (80:20v/v) with pH 5.5. Quetiapine fumarate quantified by using UV detector at 210nm. The retention time of the Quetiapine fumarate was found to be 2.6 min. The linearity of the drug concentration ranges from 20-400µg/ml. The detection and quantification limits were intended at 3.70µg/ml and 12.35µg/ml. The precision, accuracy, specificity, robustness and degradation studies were validated.

Publisher

Oriental Scientific Publishing Company

Subject

Drug Discovery,Environmental Chemistry,Biochemistry,General Chemistry

Reference17 articles.

1. International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use. ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2 (R1), Complementary Guideline on Methodology, London, 2005.

2. Nikita, G.; Archana, D.; Dubey P.K. Development and validation of assay method for determination of quetiapine fumarate by RP-HPLC from bulk and pharmaceutical dosage form. Int. J. Pharmaceutical Sciences & Medicine, 2022, 7(10), 65-96.

3. CrossRef

4. Talele, S. G; Derle, D. V. Stability-indicating high-performance liquid chromatography (HPLC) method development and validation for the determination of quetiapine fumarate in bulk and dosage form by HPLC. Int. J. Green .Pharmacy, 2018 (Suppl), 12 (1), S188-S193.

5. Rajendraprasad, N.; Basavaiah, K.; Anil Kumar, U. R. Isocratic ultra-performance liquid chromatographic assay of quetiapine fumarate in pharmaceuticals. Tha.i J. Pharm Sciences. 2017, 41 (1), 6-11.

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