Phytochemical Analysis and In vitro Anti-oxidant Activities of Medicinal Plants Cyperus rotundus, Tinospora cordifolia and their Formulation

Abstract

Free radicals are harmful macromolecules that interact with lipids, nucleic acids and carbohydrates inside cells. The high level of ROS present in the cell leads to major health issues such as diabetes, hypertension, cancer and oxidative stress. In the current study, Cyperus rotundus (C.rotundus), Tinospora cordifolia (T.cordifolia) and their formulations were evaluated for their phytochemical content, functional group identification by FT-IR and In vitro free radical scavenging activity. The phytochemical analysis showed that the ethanolic extracts had more secondary metabolites like flavonoids, alkaloids, terpenoids, and phenolic compounds than the extracts from the other solvents. Compared to the standard ascorbic acid, the ethanolic extract exhibited good free radical scavenging activity against DPPH, ABTS, hydrogen peroxide and superoxide and an elevated level of activity was observed with the increased concentration of the extract. The ethanolic extracts of C.rotundus, T.cordifolia and their formulation were investigated using FT-IR spectroscopy, which revealed the presence of unique functional groups such as primary as well as secondary amines, alkenes, nitro compounds and alkylhalides. In an In vitro model, the current finding demonstrates the anti-oxidant effectiveness of C.rotundus and T.cordifolia. Hence, further studies are warranted and it might eventually be utilized as a possible medicament for treating diseases.