Development of a Chromatographic Technique for Quantification of Drotaverine Hydrochloride and Diclofenac Potassium-Reference-Cited by-同舟云学术

Development of a Chromatographic Technique for Quantification of Drotaverine Hydrochloride and Diclofenac Potassium

Published:2023-08-30 Issue:4 Volume:39 Page:970-975
ISSN:2231-5039
Container-title:Oriental Journal Of Chemistry
language:en
Short-container-title:Orient. J. Chem

Author:

Dash Dillip Kumar¹^ORCID,Sen Ashim Kumar¹,Saisivam S.²^ORCID,Zanwar Aarti S.¹^ORCID,Patel Shivkant¹^ORCID,B. Sen Dhanya¹^ORCID

Affiliation:

1. 1Department of Pharmacy, Sumandeep Vidyapeeth Deemed to be University, At and Po. Piparia, Vadodara, Gujarat, India.

2. 2N. R. Vekaria Institute of Pharmacy, C L College campus, Bilkha road, Junagadh, Gujarat, India.

Abstract

A straightforward RP-HPLC approach has been developed and validated for the simultaneous estimation of drotaverine hydrochloride (DROTA) and diclofenac potassium (DICLO) in their combined dosage form. This method was found to be simple, accurate, and precise. Chromatographic detection has been carried out on a Phenomenex C18 column (250 mm × 4.6 mm, i.d, 5 µm) with a flow rate of 1.0 ml/min using an isocratic mobile phase consisting of Methanol and Water (80:20 v/v, pH 3 adjusted with ortho phosphoric acid). The wavelength of 280 nm was chosen for the UV detection. The separation of DROTA and DICLO took less than ten minutes, had a good resolution, and produced very little tailing. There was no interference from any excipients. The method was validated in accordance with the ICH recommendations, and the criteria for accuracy, precision, linearity, and system adaptability were all satisfactory in each and every one of the cases.

Publisher

Oriental Scientific Publishing Company

Subject

Drug Discovery,Environmental Chemistry,Biochemistry,General Chemistry

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