Purification, Characterization and N-terminal Protein Sequencing of the Enzyme Dextransucrase Produced by Leuconostoc mesenteroides

Author:

Dawoud Turki M.1ORCID,Alshehrei Fatimah2ORCID,Siddiqui Khaizran3ORCID,Ameen Fuad1,Akhtar Jameela3,Arif AfsheenORCID

Affiliation:

1. 1Department of Botany and Microbiology, College of Science, King Saud University, Riyadh11451, Saudi Arabia.

2. 2Department of Biology, Jumum college university, Umm Al-Qura University, P.O Box 7388, Makkah 21955, Saudi Arabia.

3. 3Center of Molecular Genetics, University of Karachi, Pakistan.

Abstract

Background: The wide use of dextran in many different applications, makes its industrial production a challenge and, hence, to obtain a control branched structure of this enzyme research is in progress. Objectives: In the present paper, the enzyme dextransucrase, produced by cultivation of the bacterium Leuconostoc mesenteroides CMG713, was purified and characterized. Methods: The produced dextransucrase was partially purified by PEG400 obtaining a purification factor of 29.4-fold and an overall yield of 18.3% from the initial crude enzymatic extract. Results: The partially purified dextransucrase had a specific activity of 24.0 U/mg and presented a molecular weight of about 200 kDa. In addition, the produced dextransucrase was stable at 30ºC and pH 5.5 for 3 days and led to a highly soluble dextran with wide potential industrial applications. The current study has successfully partial purification, characterization and conformation of dextransucrase produced by fermentation of the bacterium Leuconostoc mesenteroides CMG713.

Publisher

Oriental Scientific Publishing Company

Subject

Drug Discovery,Agronomy and Crop Science,Biotechnology

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