Isolation and Characterization of Diosgenin, a Phytosterol Sapogenin from the Ethanolic Root Extract of Coleus forskohlii, and its Anti-ovarian Cancer Eefficacy on the SKOV-3 Cell Line

Abstract

The successful isolation and characterization of diosgenin from the roots of C. forskohlii was accomplished by a combination of multiple analytical methods, including HPTLC, HPLC, UV, FT-IR, NMR, and mass spectroscopy. The primary goal of this research was to quantify the anticancer capabilities of the human ovarian SKOV-3 cancer cell line. The acquired compound is persisted to the preparative TLC using ethyl acetate and methanol (70:30 v/v) as eluents. Maximum absorbance was reported in the UV-Vis spectrum at 232 nm. The presence of the functional group was subsequently established by a variety of peaks in the FT-IR spectra, including 3450.06, 2947.74, 1236.38, 1054.99, and 894.11 cm-1.Structural elucidation of diosgenin had been performed through spectrum analysis for instance 13C and 1H profoundly nuclear magnetic resources. The molecular formula C27H42O3 coincides with the parent molecular ion [M+] peak at m/z 414.63 g/mol, which is apparent in the mass spectra. The presently proceeding study's findings tend to show diosgenin has an astonishing cytotoxic action (IC50: 75.47µg/ml) on human ovarian cancer cells.