Extraction, Isolation, Characterization and Nootropic Activity of Bioactive Compounds from Ethanolic Extract of Leaves of Leucaena leucocephala

Abstract

The current work aims to screen out the phytochemical screening of extract of ethanol extracted from dried leaves of Leucaena leucocephala. Specifically, the soxhlet extraction process was used, along with phytochemical analysis, separations, and the isolation of biologically active compounds using Thin Layer Chromatography (TLC), column chromatography and HPTLC, respectively. The isolated biologically active compound was then characterized using a variety of spectral analysis namely Infra-Red (IR), 1H nuclear magnetic resonance (1HNMR), 13C nuclear magnetic resonance (13CNMR), and mass spectroscopy. Phytochemicals such as carbohydrates, alkaloids, saponins, tannins, flavonoids, lipids, fixed oils, and phenols are found in the plant's ethanolic leaf extract. Spectroscopic methods were used to characterize the single isolated compound, and the findings presented that the structure of isolated compound LLQ was quercetin. Nootropic action was demonstrated by the secondary metabolites present in the ethanolic extract.