Phytochemical Screening and In Vitro Cytotoxicity of Sphagnum sericeum against Human Acute Lymphoblastic Leukaemia Cell Lines-Reference-Cited by-同舟云学术

Phytochemical Screening and In Vitro Cytotoxicity of Sphagnum sericeum against Human Acute Lymphoblastic Leukaemia Cell Lines

Published:2024-04-30 Issue:2 Volume:40 Page:333-341
ISSN:2231-5039
Container-title:Oriental Journal Of Chemistry
language:en
Short-container-title:Orient. J. Chem

Author:

Latif Elda Surhaida¹^ORCID,Awang Normah¹^ORCID,Rasli Nur Rasyiqin¹^ORCID,Xuan Daphne Wong Qiao¹,Juferi Noor Eszrezad²,Kamil Nik Norhazrina Nik Mohd³,Hamid Asmah¹^ORCID

Affiliation:

1. 1Center for Toxicology and Health Risk Studies (CORE), Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.

2. 2Institute of Medical Science Technology (MESTECH), Universiti Kuala Lumpur, Taman Kajang Sentral, 43000 Kajang, Selangor, Malaysia.

3. 3Moss Herbarium (UKMB), School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor.

Abstract

The potential of Sphagnum sericeum (SS) moss extracts as an alternative treatment for childhood acute lymphoblastic leukaemia (ALL) was investigated in this study. Aqueous, methanol, and chloroform SS extracts were analyzed for phytochemical composition and cytotoxic effects on T-cell ALL lines (Jurkat and CCL-119). Results show that the S. sericeum aqueous extract (SSAE) yielded the highest percentage, followed by the methanol extract (SSME) and the chloroform extract (SSCE). Phytochemical analysis identified alkaloids, glycosides, and terpenoids in all extracts. SSME demonstrated significant cytotoxicity towards ALL cells, with the lowest IC50 values observed at 72 hours. Notably, SSME induced morphological changes, including blebbing and cell fragmentation after 6 hours, indicative of apoptosis and cell destruction. These findings suggest SSME's potential as a natural agent for treating paediatric ALL, offering a novel approach in cancer research and contributing insights into the phytochemical compounds and cytotoxic effects of S. sericeum extracts on T-ALL cell lines.

Publisher

Oriental Scientific Publishing Company

Reference49 articles.

1. 1. Zhang P.Y., Yang Y.J., Fu C.M., Xiang L.L., Wang Q. and Li X.L., Eur Rev Med Pharmacol Sci., 2015; 19:1356-63.

2. 2. American Cancer Society. Last Medical Review: Available at: https://www.cancer.org/cancer/leukemia-in-children/.

3. 3. Hunger S.P. and Mullighan C.G., N Engl J Med., 2015; 373:1541-52. DOI: 10.1056/NEJMra1400972.

4. 4. Arakawa A., Springer., 2020: 123-139. DOI 10.1007/978-981-15-0548-5_13.

5. 5. Cooper S.L. and Brown P.A., Pediatr Clin North Am., 2015; 62: 61-73. DOI 10.1016/j.pcl.2014.09.006.