Peculiarities of Action of Catecholamines and their Metabolites in the Regulation of Cardiomyocyte Enzymes

Abstract

Абстрактный Background: Myocardial ischemia is accompanied by a significant increase in adrenaline content in the heart. By its nature, sympathetic hyperactivation is accompanied by increased formation of products of enzymatic and non-enzymatic metabolism of adrenaline and its analogues, which can change the use of ATP by cells, change the activity of mitochondrial and cytosolic enzymes, contributing to disruption of bioenergetic adaptation, antioxidant defense system and levels of intercellular modulators such as AMP and adenosine. The study objective was to explore the features of adrenaline and its analogues in the regulation of the activity of mitochondrial and cytoplasmic enzymes of cardiomyocytes. Methods: The experiment was carried out on 65 three-month-old male Wistar rats weighing 60-190 g. To study the effects of catecholamines and their metabolites in the regulation of mitochondrial and cytoplasmic enzymes activity of cardiomyocytes, experimental rats were put to death by intraperitoneal injection of 10% ketamine in an amount of 0.25 mg per 100 g. Activity of mitochondrial succinate dehydrogenase, cytochrome c oxidase, mitochondrial DNP-activated ATPase, adenosine deaminase (AD), AMP deaminase (AMPD), glutathione reductase (GR) and glutathione peroxidase (GPO) were determined. Results: Dopamine has the greatest activating effect on cardiac mitochondrial ADH, adrenaline on CHO, and adrenochrome and adrenoxyl on ATPase. Isadrine and dopamine reduce cardiac AMPase activity. An activating effect on cardiac mitochondrial AMP deaminase was found only in norepinephrine.  Заключение. В кардиомиоцитах адреналин активирует цитозольные ферменты метаболизма пуриновых нуклеотидов AD и AMPD, а также повышает уровень перекисного окисления липидов (MDA и DC). Это доказывает, что адреналин, воздействуя на адренорецепторные механизмы, приводит организм в состояние окислительного стресса. Гормоны-медиаторы симпато-адреналовой системы адреналин, дофамин, норадреналин, изадрин и метаболиты катехоламинов (адренохром и адреноксил), изменяют активность ферментов митохондриальной дыхательной цепи кардиомиоцитов, а также регулируют процессы тканевого дыхания, переводя митохондрии в состояние «рыхлого» состояния. «сочетание дыхания и фосфорилирования.