The Effect of Liposomes Ethanol Extract of Tekelan Leaves (Chromolaena odorata L.) on the SOD and MDA Level in Streptozotocin-Nicotinamide-Induced Diabetic Rats

Abstract

BACKGROUND: Oxidative stress occurs when the quantity of free radical molecules in the body exceeds the number of antioxidant molecules. Tekelan leaf (Chromolaena odorata L.) is an Asteraceae plant with antioxidant qualities that aid in the prevention of oxidative stress. According to, the chemical components contained in tekelan leaves, such as flavones, flavonols, flavonones, chalcones, and hydroxy benzoic acid, are responsible for the antioxidant properties. Tekelan leaf ethanol extract liposomes is one of preparation that can improve the level antioxidant activity of the samples, in the recent research antioxidant activity of liposomes ethanol extract of tekelan leaves (Chromolaena odorata L.) is tested to male rats which induced by Streptozotocin-Nicotinamid than calcilate the level of  SOD and MDA.     AIM: The goal of this study was to see if tekelan leaf ethanol extract liposomes might reduce MDA and SOD levels in male rats caused by STZ-nicotinamide.   METHOS: Rats were given nicotinamide at a dose of 24 mg/200gBW and streptozotocin at a dose of 12 mg/200gBW at day 1, then continue to liposome and extract administration at doses of 20 mg/200gBW, 40 mg/200gBW, and 80 mg/200gBW, respectively for 15 days. At day 16 the blood were drawn and SOD and MDA levels were analyzed using a spectrophotometer.   RESULTS:  The MDA value for liposome preparation at a dose of 80 mg/200gBW were substantially lower (9.81±0.08) than negative control group (15.94±0.45). Furthermore, similar result also reported in SOD value that liposome preparation at a dose of 80 mg/200gBW (89.33±0.30) that higher than negative control group (65.55±0.30).   CONCLUSION: It concluded that the liposomes preparation of the ethanol extract of tekelan leaves showed higher antioxidant activity than extract preparation, it showed dose dependent manner.