Evaluation of Antimicrobial and Proliferation of Fibroblast Cells Activities of Citrus Essential Oils

Author:

Syafri Suryati,Husni Elidahanum,Wafiqah Nurul,Ramadhan Fitrah,Ramadani Sovia,Hamidi DachriyanusORCID

Abstract

BACKGROUND: Citrus species produce essential oils (EOs) containing various chemical components that show many pharmacological activities. AIM: The purpose of this study is to evaluate the chemical content and antimicrobial activity of EO extracted from fruit peels and leaves of Citrus x aurantifolia (“Asam sundai”) and Citrus aurantifolia (lime EOs extracted from fruit peels and leaves). METHODS: The EO was extracted by the hydrodistillation method. The chemical content was determined using gas chromatography in tandem with mass spectroscopy (GC-MS). Antibacterial activity was performed using broth microdilution method, while proliferation of fibroblast cell was carried out using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. RESULTS: The main constituent of the EO of “asam sundai” peel (MAKS) and lime peel (MAKN) was I-limonene, while the EO of “asam sundai” leaves (MADS) was γ-terpinene. MAKN EOs showed stronger antibacterial activity than MAKS and MADS with minimum inhibitory concentration values of 3.12 mg/ml against S. aureus, MRSA, and Pseudomonas aeruginosa and 6.25 mg/ml for Streptococcus mutans and Escherichia coli. Meanwhile, the highest fibroblast cell proliferation activity showed by MAKS EO at concentrations of 10, 1, and 0.1 g/ml with a percentage of the proliferation of more than 100%. CONCLUSION: It can be concluded that the different species of citrus have different chemical compositions and different biological activities.

Publisher

Scientific Foundation SPIROSKI

Subject

General Medicine

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