Induction of Ovulation using Low-Level Laser Biostimulation

Abstract

Abstract Aim: Low level laser therapy (LLLT) has been used in biostimulation of rat ovaries using a fibreoptic laser introduced directly through the vagina to deliver laser doses, which is a new modality of laser induction of ovulation, compared with conventional medical induction of ovulation using clomiphene citrate and with non-interventional controls.   Study design: A Prospective experimental study carried out from January 2014 to February 2016. Setting: University-based photobiology laboratory. Materials and methods:  Seventy-two (72) Female-Wistar Albino rats were used in this study, divided into three groups: 17 rats used as a control group, 19 rats received clomiphene citrate (Clomid group) and 36 rats exposed to diode laser 660 nm wavelength (laser group). Histopathological assessment of rat ovaries was done in the three study Groups. Results: Higher number of follicles was found in laser group compared to controls, the difference was significant in the Primordial follicles. Non-significant increase in the number of Primordial follicles in laser group was found compared to the clomid group. Clomid group showed a non-significant increase in primary, secondary and Antral follicles. Conclusion: This study shows that ovarian laser biostimulation is a new encouraging method for induction of ovulation.