Effect of Different Solvent on Phytochemical Content, Tyrosinase Inhibition and Antioxidant Activities of Campolay (Pouteria campechiana kunth. [Baehni.])

Abstract

BACKGROUND: Pouteria campechiana leaves are reported to have phenol and flavonoid compounds. Phenolic and flavonoid compounds can act as tyrosinase inhibitor and antioxidant. AIM: The purpose of this study was to compare phytochemical content, tyrosinase inhibition, antioxidant activities, and determine of marker compound from P. campechiana leaves extract with different polarities solvent. METHODS: In addition, the content of marker compound from P. campechaina leaves extract was determined by HPLC. RESULTS: The highest total phenolic content (TPC) 7.83 GAE/100 g extract, IC50 of tyrosinase 171.512 ± 1.352 and IC50 of DPPH 0.968 ± 0.008 was given by ethanolic extract (DE). Meanwhile ethyl acetate extract (DEA) had the highest of total flavonoid content 2.544 ± 0.554 QEA/100 g extract. The TPC correlated with tyrosinase inhibitory activity and antioxidant activity. CONCLUSION: Quercitrin was as marker compound from P. campechiana leaves extract, and quercitrin content in the DEA of P. campechiana leaves was 3.539%, while in the DE was 0.153%.