Reduction of endoglin receptor impairs mononuclear cell-migration-Reference-Cited by-同舟云学术

Reduction of endoglin receptor impairs mononuclear cell-migration

Published:2020-05-22 Issue:3 Volume:1 Page:136-148
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Container-title:Exploration of Medicine
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Author:

Han Zhenying¹,Shaligram Sonali¹,Faughnan Marie E.²,Clark Dewi³,Sun Zhengda⁴,Su Hua¹

Affiliation:

1. Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA 94143, USA; Center for Cerebrovascular Research, University of California, San Francisco, CA 94143, USA

2. Toronto HHT Centre, Division of Respirology, Department of Medicine, St. Michael’s Hospital, University of Toronto, Ontario M5B 1W8, Canada; Li Ka Shing Knowledge Institute, St. Michael’s Hospital, University of Toronto, Ontario M5B 1W8, Canada

3. Toronto HHT Centre, Division of Respirology, Department of Medicine, St. Michael’s Hospital, University of Toronto, Ontario M5B 1W8, Canada

4. Department of Radiology, University of California, San Francisco, CA 94143, USA

Abstract

Aim: To test if the impairment of mononuclear cell (MNC) migration in patients with hereditary hemorrhagic telangiectasia (HHT) is due to the reduction of the endoglin (ENG) receptor on the cell surface and oxidative stress. Method: MNCs of HHT patients and normal controls were subjected to migration assay. Fractions of MNCs were pre-incubated with antibodies specific to HHT causative genes ENG [hereditary hemorrhagic telangiectasia type 1 (HHT1)] or activin receptor-like kinase 1 [ALK1, hereditary hemorrhagic telangiectasia type 2 (HHT2)], AMD3100 or Diprotin-A to block ENG, ALK1 C-X-C chemokine receptor 4 (CXCR4) or CD26 (increased in HHT1 MNCs) before migration assay. The MNCs were allowed to migrate toward stromal cell-derived factor-1alpha (SDF-1alpha) for 18 h. The expression of CXCR4, CD26, superoxide dismutase 1 (SOD1) and glutathione peroxidase 1 (GPX1) in MNCs and nitric oxide levels in the plasma were analyzed. Results: Compared to the controls, fewer HHT1 MNCs and similar number of HHT2 MNCs migrated toward SDF-1. Diprotin-A pre-treatment improved HHT1 MNC-migration, but had no effect on normal and HHT2 MNCs. Pre-incubation with an anti-ENG antibody reduced the migration of normal MNCs. Diprotin-A did not improve the migration of ENG antibody pre-treated MNCs. Anti-ALK1 antibody had no effect on MNC-migration. AMD3100 treatment reduced normal and HHT MNC-migration. ENG mRNA level was reduced in HHT1 and HHT2 MNCs. ALK1 mRNA was reduced in HHT2 MNCs only. CD26 expression was higher in HHT1 MNCs. Pre-treatment of MNCs with anti-ENG or anti-ALK1 antibody had no effect on CD26 and CXCR4 expression. The expression of antioxidant enzymes, SOD1, was reduced in HHT1 MNCs, which was accompanied with an increase of ROS in HHT MNCs and nitric oxide in HHT1 plasma. Conclusions: Reduction of ENG receptor on MNC surface reduced monocyte migration toward SDF-1alpha independent of CD26 expression. Increased oxidative stress could alter HHT MNC migration behavior.

Funder

National Institutes of Health

Publisher

Open Exploration Publishing

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