Intestinal radiocalcium absorption in the goat: measurement by a double-isotope technique

Abstract

1. Intestinal radiocalcium absorption was measured in goats by a double-isotope technique involving injection of45CaCl2intravenously and47CaCl2into the abomasum. Cumulative absorption of radiocalcium was calculated by deconvolution analysis form curves of plasma radioactivity.2. Repeated measurements at 2 d intervals gave highly reproducible results (r0.94,P< 0.001). No systematic difference between two consecutive measurements was observed. A good agreement between absorption of radiocalcium from simultaneously administered47CaCl2and45Ca-labelled hay (r0.93,P< 0.001) seems to justify the use of inorganic47Ca as a tracer for Ca in ruminant diets.3. Two- to three-fold increases in radiocalcium absorption 48 h after oral treatment with 1,25- dihydroxycholecalciferol or leaves ofSolanum malacoxylonshowed the usefulness of the method in situations of rapidly changing Ca absorption.4. Endogenous adaptations in intestinal radiocalcium absorption from 20 to 43% were observed in lactating goats when Ca intakes decreased from 12 to 4 g/d.5. It is concluded that the double-isotope technique is a suitable method for studies of Ca absorption in ruminants when tracer is introduced into the abomasum. The test is completed in 3–4 h and may therefore be used in situations where the absorption of Ca undergoes rapid changes.