The effect of high-protein and high-carbohydrate diets on [125I]iodoinsulin binding in skeletal muscle plasma membranes and isolated hepatocytes of rainbow trout (Salmo gairdneri)

Abstract

1. [125I]iodoinsulin-binding studies in the presence of a concentration range of bovine insulin were conducted to establish specific insulin-binding levels in skeletal muscle plasma membranes and isolated hepatocytes of rainbow trout (Salmo gairdneri) reared on control, high-protein or high-carbohydrate diets.2. Negative co-operativity was observed and receptor concentrations and apparent dissociation constants established for each preparation.3. No differences of specific binding attributed to diet were detected in skeletal muscle plasma membrane preparations; however, the receptor concentration of isolated hepatocytes from high-carbohydrate-reared trout was increased. This contrasted to comparable mammalian studies.