Influence of foliage from African multipurpose trees on activity of rumen protozoa and bacteria

Abstract

Samples and extracts of foliage from African multipurpose trees were screened for their effects on rumen protozoa and bacteria with a view to predicting their safety as feed supplements and for identifying species with potential antiprotozoal activity. The species tested wereAcacia aneura, Charnaecytisus palmensis, Brachychiton populneum, Flindersia maculosa, Sesbania sesban, Leucaena leucocephalaandVernonia amyedalina. Antimicrobial effects were mild except forS. sesban, which was highly toxic to rumen protozoain vitro, andA. aneura, which was toxic to rumen bacteria. The antiprotozoal factor inS. sesbanwas apparently associated with the fraction of the plant containing saponins. WhenS. sesbanwas fed to sheep, protozoal numbers fell by 60 % after 4 d, but the population recovered after a further 10 d.In vitroexperiments demonstrated that washed protozoa from later times were no more resistant toS. sesbanthan on initial exposure, suggesting that other micro-organisms, probably the bacteria, adapted to detoxify the antiprotozoal agent. ThusS. sesbanmay be useful in suppressing protozoa and thereby improving protein flow from the rumen, but only if the bacterial metabolism of the antiprotozoal factor can be avoided.