AbstractThis chapter cover topics on: procedures for gynogenic embryo induction (choice of organ and culture procedure; flower bud developmental stage; cultivation of donor plants; sterilization of explants and temperature treatments); media composition (basal mineral components; carbohydrates and gelling agents; plant growth regulators); genotypic effect; gynogenic haploid induction processes in onion; determination of ploidy and homozygosity; genome-doubling procedures and fertility; genetic stability of regenerants; and the use of doubled-haploids in onion breeding and basic research.