Plant genotyping by analysis of microsatellites.

Abstract

This chapter covers the following: isolation of simple sequence repeats (SSRs); detection of SSR amplification products; allele sizing artefacts; physical mapping of SSRs; advantages and disadvantages of SSRs over other markers; use of SSRs in genetic mapping; isolation of SSRs from barley and wheat; and applications of SSRs in the identification of barley and wheat cultivars.